Press Release / Health and Fitness / Plasmid titration can be a valuable technique for the optimization and enhancement of soluble protein expression in mammalian cells

Plasmid titration can be a valuable technique for the optimization and enhancement of soluble protein expression in mammalian cells

While DNA-mediated transient transfection of mammalian cells is a widely-used method for recombinant protein production, it is not clear as to what the optimal concentration of DNA should be. Using Nod-like receptors (NLRs) as a case study, a group of scientists demonstrated that 50-fold plasmid dilution increased the amount of soluble protein nearly 5-fold.

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