Press Release / Health and Fitness / E.coli mRNA breakdown results in dramatic improvements in recombinant protein production

E.coli mRNA breakdown results in dramatic improvements in recombinant protein production

MazF is an E.coli enzyme that degrades cellular mRNA in a targeted fashion, at the "ACA" sequence. By modifying all "ACA" triplets in the target gene, a study showed that induction of MazF toxin caused degradation of E.coli cellular mRNA but the recombinant gene transcription and protein synthesis continued, causing significant accumulation of high quality target protein.


For years, GenScript
has been the industry leader in synthetic DNA technology. As performed in this study, we codon-optimize

the target gene to eliminate any unnecessary sequence elements which also helps achieve high levels of soluble expression. With

its robust gene-to-protein service offerings, GenScript will be a reliable partner throughout your protein production campaign.




BacPower™ Customized service C what’s included
> State-of-the-art OptimumGene™ gene design technology
> Sub-cloning and expression optimization
> Pilot scale protein expression and purification
> Protein characterization